Protein Engineering and Design

Our Approach to Protein Synthesis

HarkerBIO offers a next-generation business model for supporting structure accelerated lead discovery. We have built a team and a culture dedicated to helping your drug discovery process. Equally important, HarkerBIO is investing in innovation and the latest technology and techniques in the field of structure biology. We execute the highest quality platforms of our partners and enable their proprietary technologies with embedded full-time equivalent (FTE) and expertise.

Our experience includes a deep knowledge of the discovery and development work done on many undruggable targets, including: 

  • G-protein-coupled receptors (GPCRs), ion channels, and membrane proteins 
  • Kinaseswith or without conjugated strategiesand nanoparticle work 
  • Epigenetic targets, including bromodomains and HDACs  
  • Difficult protein classes such as PDEs, proteases, gyrases, HIV complexes
  • Mammalian and insect E. coli protein expression
  • Biologics such as Fabs, FAB-AG, Fc, or full-length antibody structures to compare to a biosimilar

Targeted Protein Types

ABL1, ACK1, ALK, Aurora-A, Aurora-B, BMX, B-Raf, BTK, CDK8/cyclin-C, CHK1, c-Kit, CLK2, c-Met, CDK2, eEF2 kinase, EGFR, EPHA4, EPHB4, ERK2 (MAPK1), ERK5 (MAPK7), FAK1 (PTK2), FES, FGFR4, FLT3, glucokinase, GSK3B, HPK1, IGF1R, insulin receptor, IRAK4, IRE1α, ITK, JAK1, JAK2, JAK3, JNK1, KDR (VEGFR2), LCK, LIMK2a, MAP3K12, MAPKAPK2, MAPK14 (p38a), MEK1 (MAP2K1), MEK2, MNK2, MST1, NEK2, NIK, PAK1, PAK4, PERK1, PI3Kα, PI3Kγ, PI3Kδ, PI4Kß, PIM1, PIM2, PKACα, PKB (AKT1), PKCα, PKCθ, PKCι, PLK2, PLK4, PYK2, RC kinase, RET, ROCK1, ROCK2, RON, RSK2, ROS1, sphingosine kinase, SRC, STK33, SYK, TAK1 (MAP3K7 TAB1), TBK1, TIE2 (TEK), TRKA, TYK2

To aid the transfer of phosphate groups from ATP molecules to specified molecules, a kinase is used to catalyze the reaction, and are useful for the research of cellular pathways and metabolism.

BRD4 (Bromo1), DNMT1, DNMT3B/3L, DOT1L, G9a, HDAC4, JMJD2A, JMJD2C, KEAP1, KDM2A, LSD1, UCHL1, USP2, USP7, USP8, USP14

These molecules do not originate directly in the DNA sequence of an cell, but rather are environmental or external factors that affect the DNA without altering its nucleotide sequence. This is useful for studying the direct effects of an external change to DNA, such as in twin studies, genomic imprinting research, and cancer research.

BCL2, BCL6, BCLX, MCL1, MDM2, MDM4

Apoptosis regulators are molecules that dictate the process of programmed cell death (PCD), and form a key component of cancer and tumorigenesis research.

ADAMTS5, BACE1, BACE2, BACE2-Xaperone, calpain-1 (CAPN1), caspase1, caspase-6, caspase-9, cathepsin D, cathepsin H, cathepsin K, cathepsin L1, chymase, DPP IV, DPP VIII, elastase-2, Factor VIIa, Factor IXa, Factor Xa, Factor XIa, FAPα, HCV NS3/4A protease, HIV1 protease, MMP2, MMP9, MMP12, MMP13, PCSK9, proteasomes, thrombin

Protease are enzymes that catalyze the breakdown of other proteins into smaller polypeptides and amino acids. They are a core part of protein research itself as well as industrial applications.

PDE1B, PDE2A, PDE4A, PDE4B, PDE4D, PDE7A, PDE10A

The study of phosphodiesterases, or PDEs, has numerous therapeutic applications as well as ongoing research into further applicability, with PDE inhibitors being used in the treatment of various conditions such as coronary heart disease, seizures, schizophrenia, depression, protozoal infections like malaria, and dementia.

ACC2 (CTD), aldose reductase, ß-catenin, car toxin, cyclophilin D (PPIase F), DHODH, DNA topoisomerase 1, FAAH, FASN, gag-pol polyprotein, γ-glutamyltranspeptidase, gyrase A, gyrase B, HCV NS5A domain I, HCV NS5B polymerase, HSA, HSD11B1, HSP90α, IDO1, IL17A, KIF11 (KSP/EC5), K-Ras, LXRα, LXRß, MTH1, NS5 polymerase (DENV), PARP1, PPARγ, PPARδ, TDO (Dm), TGM2, TNFα

HarkerBIO is also skilled in the production of other types of enzymes.

HarkerBio Provides Expert Structure Determination Services

Our staff has the combined decades of applied experience needed for obtaining pure protein for high quality crystals and determining protein structure to help elucidate function. Decision-making based on a sound knowledge of structure biology is critically dependent on time, so not reinventing the wheel and getting critical data on the first experiment is most important to us. Our areas of critical knowledge and successful case studies include strategic approaches using such techniques as:

  • Protein refolding
  • Large scale mutagenesis
  • Computational modeling focused on allosteric protein interactions
  • Optimized expression systems
  • Micro-purifcation protocols
  • Fragment based library screening

Contact us today for more information, or schedule an appointment with our monthly crystallization screening.

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Monthly Crystallization Screening